A Soybean Seed Transcription Factor RNAi Knock-Out Collection
To study the functions of transcription factor genes active during soybean seed development, we collaborated with Dr. David Somers (Monsanto) to generate a collection of soybean seed RNAi knock-out lines. We used the CaMV 35S gene promoter to generate RNAi lines for 63 transcription factor genes that are expressed in specific seed regions at the globular, heart, cotyledon, and early-maturation stages of development. RNAi transgenes were integrated into the soybean genome, and R0 lines containing a single RNAi transgene were isolated and grown to maturity in the greenhouse. The developing R1 and R2 seed populations were screened for developmental abnormalities associated with seed and vegetative development. A preliminary screen yielded three lines with significant phenotypes. The remainder appeared similar to wild type under our screening conditions. A list of each transcription factor gene knock-out line, their seed expression profile, and RNAi phenotype is presented in the table below.
This work had been published on Plant Physiology (click here to read the manuscript). If you have used our data, please cite the manuscript using the following reference:
John Danzer, Eric Mellott, Anhthu Q. Bui, Brandon H. Le, Patrick Martin, Meryl Hashimoto, Jeanett Perez-Lesher, Min Chen, Julie M. Pelletier, David A. Somers, Robert B. Goldberg and John J. Harada, Down-Regulating the Expression of 53 Soybean Transcription Factor Genes Uncovers a Role for SPEECHLESS in Initiating Stomatal Cell Lineages during Embryo Development, Plant Physiolgy 2015 Jul;168(3):1025-35. doi: 10.1104/pp.15.00432.
Phenotypes of Some RNAi Knock-Out Lines (click on image to enlarge)
A Complete Summary of RNAi Knock-Out Lines (click on image to enlarge)
To find the expression profile of target genes during seed development, first go to the "Browse Soybean mRNAs Profiling Database" page. Next, type the target gene name (e.g. Glyma04g41710), in the "Predicted Gene Model ID" window and click the "Submit Query" button to search the database. Lastly, in the search results page, click on the probe set corresponding to the target gene to view the expression profile.